Study the Effect of Hydrogen Peroxide and Alpha Lipoic Acid on Wound Healing in Rabbits

The aims of this study was to compare between the effect of hydrogen peroxide (oxidant) and lipoic acid (Antioxidant) on wound healing in rabbits. Fifteen adult healthy rabbits were used. It is divided in to three groups , control group, and hydrogen peroxide group(oxidant group) and hydrogen peroxide with lipoic acid group (Anti-oxidant group), also each group divide into three equal sub group for histopathological study at 1 week , 2 week and 3 week post operation. The wound was done in the femur (thigh) after general anesthesia by blade 3 cm. post-operation, in oxidant group, the animals given orally 50mgbw of hydrogen peroxide daily for 7 days, 14 days and 21daysbut in antioxidant group, the animals were given orally 50mgbw of hydrogen peroxide and 50mgbw of Alpha Lipoic acid daily for 7days , 14days and 21days. The histopathological examination appear in one week, both groups showed ulceration but in all groups showed. In two week, control group and oxidant group showed of ulceration with dermal fibrosis. In two week, in anti oxidant group showed reduce the size ulcer with dermal fibrosis. In three week, control group and oxidant group showed complete healing with moderate thickening of epidermis and hyperkeratosis with dermal fibrosis and reduce the size of ulcer. While in anti oxidant group showed complete healing with marked dermal fibrosis but presence of numerous of hair folliculate and absent of ulceration. Conclusion the lipoic acid produced an increase the skin healing, which may be related to alteration of histopathological parameter and antioxidant activity.