化学
体内
斑马鱼
荧光
内生
体外
生物标志物
生物化学
酶
葡萄糖醛酸酶
生物物理学
量子力学
生物
基因
物理
生物技术
作者
Yinzhu Jin,Xiangge Tian,Lingling Jin,Yonglei Cui,Tao Liu,Zhenlong Yu,Xiaokui Huo,Jingnan Cui,Cheng‐Peng Sun,Chao Wang,Jing Ning,Baojing Zhang,Lei Feng
标识
DOI:10.1021/acs.analchem.7b04813
摘要
β-Glucuronidase (GLU) is an important biomarker for primary cancers and intestinal metabolism of drugs or endogenous substances; however, an effective optical probe for near-infrared (NIR) monitoring in vivo is still lacking. Herein, we design an enzyme-activated off-on NIR fluorescent probe, HC-glu, based on a hemicyanine keleton, which is conjugated with a d-glucuronic acid residue via a glycosidic bond, for the fluorescent quantification and trapping of endogenous GLU activity in vitro and in vivo. The newly developed NIR probe exhibited prominent features including prominent selectivity, high sensitivity, and ultrahigh imaging resolution. It has been successfully used to detect and image endogenous GLU in various hepatoma carcinoma cells, tumor tissues, and tumor-bearing mouse models, for cancer diagnosis and therapy. Moreover, it could detect the in vivo activity of GLU in the intestinal tracts of animals including mice and zebrafish, where GLU performs a vital biological function and is mainly distributed. It could also evaluate real intestinal distribution and real-time variations of GLU in development and growth, all of which are very helpful to guide rational drug use in the clinic. Our results fully demonstrated that HC-glu may serve as a promising tool for evaluating the biological function and process of GLU in living systems.
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