壳聚糖
点击化学
甜菜碱
化学
抗菌活性
纳米颗粒
组合化学
聚合物
硫醇
阳离子聚合
高分子化学
核化学
有机化学
材料科学
纳米技术
生物
细菌
遗传学
作者
Andreii S. Kritchenkov,Anton R. Egorov,Alexey A. Artemjev,Ilya S. Kritchenkov,О. В. Волкова,Aleh V. Kurliuk,Tatsiana V. Shakola,В. В. Рубаник,Alexander G. Tskhovrebov,Niyaz Z. Yagafarov,Victor N. Khrustalev
标识
DOI:10.1016/j.ijbiomac.2019.11.241
摘要
In this work, we demonstrate that the thiol-yne click reaction could be efficiently mediated by ultrasonic irradiation and implement the ultrasound-assisted thiol-yne click reaction to chitosan chemistry as a polymer-analogous transformation. We optimize power and frequency of ultrasound to preserve selectivity of the click reaction and avoid ultrasonic degradation of the chitosan polymer chain. Thus, we obtain a new water-soluble betaine. Using ionic gelation of the obtained betaine derivatives of chitosan, we prepare nanoparticles with a unimodal size distribution. Furthermore, we present results of antibacterial and transfection activity tests for the chitosan derivatives and their based nanoparticles. The derivative with a medium molecular weight and a high degree of substitution demonstrated the best antibacterial effect. It derived nanoparticles with a size of ca. 100 nm and ζ-potential of ca. +69 mV revealed even higher antibacterial activity, slightly superior to commercial antibiotics ampicillin and gentamicin. On the contrary, the obtained polymers possess a much more pronounced transfection activity as compared with their based nanoparticles and species with a low degree of substitution acts as the most efficient transfecting agent. Moreover, the obtained betaine chitosan derivatives as well as their derived nanoparticles are non-toxic.
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