蛋白质羰基化
启动(农业)
生物
发芽
老化
非生物成分
长寿
维格纳
休眠
园艺
植物
生物化学
酶
谷胱甘肽
生态学
遗传学
作者
Lilya Boucelha,Ouzna Abrous-Belbachir,Réda Djebbar
摘要
For a long time, it has been known that seed priming allows the improvement of plant production and tolerance to abiotic stresses. However, a negative effect on the longevity of the seeds thus primed was observed; these mechanisms are still poorly understood. In addition, it has been shown by several authors that seed ageing is associated with the oxidation and particularly with carbonylation of protein. Our work consisted in studying the AOPP and carbonyl protein at the different parts of the embryo from freshly primed seeds and from those that have been primed for 4 years (after storage). We subjected Vigna unguiculata (L.) Walp. seeds to a single or double hydropriming. Our study showed that hydropriming, and more particularly a double cycle of hydration-dehydration, makes it possible to attenuate the oxidation of the protein while it favours a certain threshold of carbonylation in the freshly dehydrated seeds in order to better trigger the germination process. On the other hand, after a storage period of 4 years, these dehydrated seeds are characterised by a strong accumulation of the products of oxidation and especially carbonylated protein, compared with the untreated seeds, which could explain the decrease of the longevity of these seeds.
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