生物加工
3D生物打印
明胶
移植
材料科学
组织工程
纳米技术
计算机科学
生化工程
生物医学工程
化学
工程类
生物化学
医学
外科
作者
Lei Shao,Qing Gao,Chaoqi Xie,Jianzhong Fu,Meixiang Xiang,Yong He
标识
DOI:10.1002/adhm.201901142
摘要
Abstract Maintaining an adequate supply of nutrients/oxygen is a major challenge in the biofabrication of large tissue constructs. However, building preformed nutrient networks may be an effective strategy for engineering thick tissues. Here, a novel way for bioprinting large‐scale tissue constructs with intentional nutrient networks is presented. A special nozzle is developed which can print bioink and sacrificial ink half and half synchronously in a single filament. Nutrient networks of these bioprinted constructs are formed by subsequently dissolving away gelatin, which allows for effective oxygen, nutrient, and waste diffusion, facilitating the cell activity and the generation of functional tissues. Due to the cell‐laden bioink and sacrificial ink working together and promoting each other's printability to support themselves, complex soft cell‐laden constructs with nutrient networks can easily be printed. Furthermore, two different cell types (osteoblast, human umbilical vein endothelial cells) encapsulated in the bioprinted large‐scale constructs (≥1 cm) with nutrient networks show enhanced cell viability and spreading within a period of culture. It is envisioned that the advanced bioprinting technology may have significant potentials in facilitating the engineering of complex structures for tissue‐specific needs, and bioprinting large‐scale tissue constructs with nutrient networks toward applications in organ transplantation and repair.
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