牛血清白蛋白
拉曼散射
纳米颗粒
分子
化学
对接(动物)
拉曼光谱
纳米技术
马兜铃酸
色谱法
材料科学
组合化学
有机化学
光学
医学
护理部
生物
遗传学
物理
作者
Yun Gao,Tong Xuan,Cuicui Fu,Yiping Wu,Xiaoyu Guo,Ying Wen,Haifeng Yang
标识
DOI:10.1016/j.snb.2019.127223
摘要
As one of the most potent carcinogens, aristolochic acid I (AAI) can be founded in many plants, which are widely used as raw materials in traditional Chinese medicine. Surface enhanced Raman scattering (SERS) method would be developed to detect the AAI in rapid way. However, hydrophobic AAI molecule with small scattering section hardly approaches onto the surface of metal nanoparticles, resulting in poor Raman detection sensitivity. In this work, bovine serum albumin (BSA) decorated silver nanoparticles (Ag NPs) with core-shell structure ([email protected] NPs) are successfully synthesized. The molecular docking induced strong interaction between BSA and AAI is validated by molecular simulation and [email protected] NPs could actively capture AAI and anchor the target molecule into the hot spots of Ag NPs, which greatly improves SERS response of AAI. The dynamic linear range for SERS detection of AAI is from 0.5 to 50 μM (R2 = 0.9910) and the lowest detectable concentration of 0.28 μM is reached, which meeting the strict limitation of national standard regulation for AAI in cosmetic products. In addition, the method is applied for AAI determination in skin care products and good results are achieved.
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