Vital Roles of A-Type Linkage and Polymerization Degree in Governing Proanthocyanidin Interaction and Anti-inflammatory Action on Litchi Thaumatin-Like Protein

a = 283.7 L/mol) with LcTLP. Thermodynamic parameters indicated hydrogen bonding as dominant, reflecting an enthalpy-driven process. In RAW264.7 cells, PAPC more effectively suppressed LcTLP-induced NO, TNF-α, and IL-6 secretion by coating LcTLP surface. However, oligomeric proanthocyanidins occupied LcTLP active site by binding to the key residues, including GLU84 and TYR85.