Additional file 1: Figure S1. of Pigment epithelium-derived factor alleviates endothelial injury by inhibiting Wnt/β-catenin pathway

Ox-LDL induces apoptosis in HUVECs. HUVECs were treated with ox-LDL at different concentrations (12.5, 25, 50, 100 and 150 mg/L) for 24 h, and cell apoptosis was detected by SYTO-13/PI double-staining. Representative fluorescence images and quantitative data are shown. Green, SYTO-13-positive cells; red, apoptotic cells stained by PI. Scale bar =100 μm. The results are shown as the mean ± SD of 6 independent experiments. * P < 0.05, ** P < 0.01 versus the control group. (PDF 243 kb)