Preparation of ROS-responsive baicalin liposomes and evaluation of their therapeutic effects on hemorrhagic stroke-induced brain injury

using drug loading, particle size, morphology, release behavior, and encapsulation efficiency. Under high ROS conditions, internalization of ROS-responsive (DSPE-SS-PEG-modified) formulation was improved based on findings of cellular uptake studies. At the ischemic site, liposomes promoted targeted accumulation and prolonged systemic circulation of the drug. Neurological deficits, cerebral edema, and neuronal apoptosis were significantly alleviated in a rat model of hemorrhagic stroke by the ROS-responsive liposomes, which outperformed conventional liposomes and free Ba. Synergistic regulation of inflammatory and oxidative stress pathways mediated these therapeutic benefits. Altogether, the therapeutic efficacy of BA in hemorrhagic stroke can be effectively enhanced by this ROS-responsive (stimuli-responsive) liposomal system.