葡萄酒
亚硫酸盐
检出限
白葡萄酒
化学
微流控
色谱法
毛细管作用
比色法
有机化学
纳米技术
材料科学
复合材料
食品科学
作者
Manuel J. Arroyo,Ignacio de Orbe-Payá,Mariano Ortega‐Muñoz,Jose Vilar-Tenorio,David Gallego,Gerhard J. Mohr,L.F. Capitán-Vallvey,Miguel M. Erenas
标识
DOI:10.1016/j.snb.2022.131549
摘要
A microfluidic paper-based analytical device integrating a chromoreactand – a formylazo dye– has been fabricated and used for a colorimetric assay of sulfites. The chromoreactand was covalently linked to paper by vinyl sulfone chemistry. This work presents two robust capillary microfluidic devices to determine sulfite in wine without any pretreatment. One of them based on thread (µTPAD) useful to determine it in white wine and another based on paper (µPAD) to specifically determine sulfite in red wine as well as in white wine. Both are based on the selective recognition of sulfite by means of a chromoreactand that turns from orange to yellow in the presence of sulfite. The colour information acquired (H coordinate) using a digital camera readout allows for a range of application of the µTPAD from 7.8·10−5 M (8.1 mg L−1) to 2.7·10−3 M (279.3 mg L−1) with a limit of detection (LOD) of 78 µM. The strong interference caused by the dyes present in red wine is eliminated by including a laminated paper channel in the µPAD structure that allows for the separation of colorants from red wine before the recognition of the sulfite. This makes it possible to adjust the µPAD procedure to the usual sulfite concentration in wine, with an LOD of 2.2·10−4 M (22.7 mg L−1) and a CV of 2.6%.
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