体内
体内分布
肿瘤缺氧
材料科学
生物物理学
缺氧(环境)
荧光寿命成像显微镜
药物输送
体外
活力测定
癌症研究
生物医学工程
荧光
纳米技术
化学
生物
生物化学
医学
氧气
放射治疗
有机化学
生物技术
内科学
量子力学
物理
作者
Yun Zeng,Peng Chang,Jingwen Ma,Ké Li,Chunhong Zhang,Yingying Guo,Hanrui Li,Qingxia Zhu,Huifang Liu,Wenjing Wang,Yuwei Chen,Dan Chen,Xu Cao,Yonghua Zhan
标识
DOI:10.1021/acsami.1c22620
摘要
Hypoxia is a well-known feature of malignant solid tumors. To explain the misinterpretation of tumor hypoxia variation during chemotherapy, we developed a DNA origami-based theranostic nanoplatform with an intercalated anticancer anthraquinone as both the chemotherapeutic drug and the photoacoustic contrast agent. The size distribution of the DNA origami nanostructure is 44.5 ± 2.3 nm, whereas the encapsulation efficiency of the drug is 90.7 ± 1.0%, and the drug loading content is 92.2 ± 0.1%. The controlled cumulative release rates were measured in vitro, showing an acidic environment induced rapid drug release. The values of free energy of binding between the drugs and the DNA double helix were calculated through molecular simulations. The cell viability assay was used to characterize cytotoxicity, and fluorescence confocal cell imaging illustrates the biodistribution of the probe in vitro. Photoacoustic and fluorescence imaging were used to indicate drug delivery, release, and biodistribution to predict the drug's chemotherapeutic effect in vivo, whereas the photoacoustic signals were compared with those of deoxygenated/oxygenated hemoglobin to represent the tissue hypoxia/normoxia maps during the chemotherapeutic process and indicate alleviated tumor hypoxia. Staining of tissue sections taken from organs and tumors was used to verify the results of photoacoustic imaging. Our results suggest that photoacoustic imaging can visualize this DNA origami-based theranostic nanoplatform and reveal the mechanisms of chemotherapy on tumor hypoxia.
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