医学
外周血单个核细胞
免疫学
自身免疫性疾病
红斑狼疮
生物标志物
内科学
疾病
内分泌学
生物
体外
生物化学
抗体
作者
Chuen‐Miin Leu,Tzu-Sheng Hsu,Yu‐Ping Kuo,Ming‐Zong Lai,Po-Chun Liu,Ming-Huang Chen,Deh‐Ming Chang,Chang‐Youh Tsai,Ming‐Han Chen
出处
期刊:Rheumatology
[Oxford University Press]
日期:2019-01-09
卷期号:58 (4): 719-728
被引量:5
标识
DOI:10.1093/rheumatology/key418
摘要
Abstract Objective Deletion of Deltex1 (DTX1) in mice caused hyperactivation of T cells and lupus-like autoimmune syndromes, however, the association of DTX1 with human autoimmune diseases is totally unknown. This study investigated the role of DTX1 in human T cell functions and its correlation with disease activity in patients with SLE. Methods The influence of DTX1 on T cell function was evaluated using human primary cells. DTX1 expression in peripheral blood mononuclear cells (PBMCs) from healthy controls and SLE patients was measured by quantitative real-time PCR and the SLEDAI was used to assess disease activity. Results After stimulation with anti-CD3 and anti-CD28, silencing of DTX1 expression enhanced IFN-γ secretion by human T cells. The expression of DTX1 in PBMCs was significantly lower in 100 SLE patients than in 50 age- and sex-matched healthy controls (DTX1/glyceraldehyde 3-phosphate dehydrogenase, 0.452 vs 1.269, P < 0.001). The area under the receiver operator characteristics curve of the model was 0.737 (95% CI 0.658, 0.815). Intriguingly, a low DTX1 level in T cells led to high IFN-γ production in SLE patients and had a correlation with severe disease activity. In addition, low DTX1 expression in SLE patients was associated with active LN, lung involvement or hypocomplementaemia. Conclusion Knockdown DTX1 expression in human T cells reduced IFN-γ secretion. DTX1 expression in the PBMCs was significantly lower in SLE patients and had an inverse correlation with disease activity, indicating that the DTX1 level may be a good disease marker of SLE.
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