化学
分析物
适体
生物传感器
多路复用
过氧化物酶
分子印迹聚合物
纳米技术
组合化学
色谱法
酶
生物化学
分子生物学
生物信息学
选择性
生物
催化作用
材料科学
作者
Xiaoyu Wang,Qin Li,Min Zhou,Zhangping Lou,Hui Wei
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2018-09-03
卷期号:90 (19): 11696-11702
被引量:147
标识
DOI:10.1021/acs.analchem.8b03374
摘要
Nanozymes have emerged as promising alternatives to overcome the high cost and low stability of natural enzymes. Nanozymes with peroxidase-like activities have been studied to construct versatile biosensors by using specific biorecognition ligands (such as enzymes, antibodies, and aptamers) or molecularly imprinted polymers (MIPs). However, the use of bioligands compromises the high stability and low cost promise of nanozymes, while the MIPs may not be applicable to multiplex detection. To address these limitations, here we constructed the nanozyme sensor arrays based on peroxidase-like Pt, Ru, and Ir nanozymes. The cross-reactive nanozyme sensor arrays were successfully used for the detection of biothiols and proteins as well as the discrimination of cancer cells because of the differential nonspecific interactions between the components of the sensor arrays and the analytes. The usefulness of the nanozyme sensor arrays was further validated by the detection of blind unknown samples, where 28 of 30 biothiols and 42 of 45 proteins were correctly identified. Moreover, the practical application of the nanozyme sensor arrays was demonstrated by the successful discrimination of biothiols in serum and proteins in human urine.
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