Cloning and Sequence Analysis of the phyA and phyB Gene of Aspergillus niger WP1

In order to get a high phytase activity strain,the phyA1 and phyB1 encoding phytase of Aspergillus niger WP1 were amplified by the polymerase chain reaction(PCR)with primers designed according to the sequences in Gene Bank.The amplified fragments were cloned and sequenced.The results showed that the coding region were 1 346 bp and 1 560 bp in size,there was no intron in phyA1 gene,encodes a peptide of 448 amino acids;but phyB1 includes 3 introns,and encodes a peptide of 460 amino acid residues.The area of highest homology RHGXRXP was found in the two sequences.There were two HD in phyA1 and only one in phyB1.The phyA1 and phyB1 of A.niger WP1 strain in this paper their sequences have been accessed by Gene Bank(Accession:DQ650711;DQ836360).