In this paper,we designed a novel turn- on fluorescent probe 1 for Hg2 +detection by employing Rhodamine B as the recognition moiety. The intensity at λ = 580 nm of fluorescent probe 1 increased significantly after the coordination with Hg2 +,with a color change from light yellow to pink,allowing rapid determination of Hg2 +. The fluorescent intensity at λ = 580 nm exhibited a good linear relationship with the concentration of Hg2 +between 1 to 7 μmol/ L. A detection limit of Hg2 +as low as 34 pmol/ L was achieved by this probe,and no interference was found for the detection of Hg2 +even in the coexistence of other 8 metal ions.