Antibiotics are produced by organisms in the production process,and can selectively restrain or kill microorganisms and other cancer cells at low concentration.Antibiotics have been used to treat diseases on animals and human for a long time,and the influence of antibiotics in environment on ecology and human health has been recognized.In the environment,antibiotics may leave residue in forage with different ways,or be produced by microorganism to harm human health.It has been confirmed that the antibiotics are detected in forage.This paper discussed the sources,detection method,and effect of antibiotics in forage.