The research of a NCII design experiment for heterosis groups of thirteen exceptional maize inbred lines by Selecting the different heterosis groups and the pedigree of five inbred lines tester,thirteen maize inbred lines are divided into four heterosis groups through the cluster analysis,The result is as follows: Lancaster group(NH-14,Spx3,Dx37);Reid group(LN-2);Tangsipingtou group(LN-15,Spx56,Dx321);Lvdahonggu group(red-axis598,DY112,LD61,DY45);KS75 and KSt12 are difficult to and other inbred lines from delivering heterosis groups into one among each other of them.