Different from the traditional Cordyceps Sinensis preparation methods,the Cordyceps Sinensis rhizomorph has been used to prepare and optimize the liquid culture media of cordyceps sinensis mycelia.Results show that the optimal medium for the mycelia has 7.5%glucose,7.5%sucrose,0.12%peptone,0.375%yeast powder,0.15%KH_2PO_4,0.075% MgSO_4·7H_2O,0.015%VB_1 and natural pH.Under the cultural condition of 20℃and 192 h,the mycelia biomass may reach 4.242 g/L,and in the next Grade 2 and Grade 3 Culturing processes,the hyphae has excellent characters and grows faster.