Cyclodextrin Host‐Guest Recognition Approach for Simultaneous Quantification and Voltammetric Studies of Levodopa and Carbidopa in Pharmaceutical Products

Abstract An electrochemical sensor for simultaneous quantification of Levodopa (L‐dopa) and Carbidopa (C‐dopa) using a β ‐cyclodextrin/poly( N ‐acetylaniline) ( β ‐CD/PNAANI) modified carbon paste electrode has been developed. Preconcentrating effect of β ‐CD as well as its different inclusion complex stability with L‐dopa and C‐dopa was used to construct an electrochemical sensor for quantification of these important analytes. The overlapping anodic peaks of L‐dopa and C‐dopa at 810 mV on bare carbon paste electrode resolved in two well‐defined voltammetric peaks at 450 and 880 mV vs. Ag/AgCl, respectively, with a drastic enhancement of the anodic peak currents. Under optimized conditions, linear calibration curves were obtained in the ranges of 0.5–117 µM and 1.6–210 µM with detection limits down to 0.2 and 0.8 µM for L‐dopa and C‐dopa, respectively. The proposed electrode was successfully applied for the determination of L‐dopa /C‐dopa in pharmaceutical formulations and the results were in close agreement with the labeled values.