碘化丙啶
膜联蛋白
细胞凋亡
冷库
高架桥
坏死
程序性细胞死亡
低温保存
细胞生物学
男科
化学
生物
生物化学
医学
内科学
园艺
胚胎
作者
Aby J. Mathew,Robert G. Van Buskirk,John G. Baust
出处
期刊:Cell Preservation Technology
[Mary Ann Liebert]
日期:2002-12-01
卷期号:1 (4): 239-253
被引量:33
标识
DOI:10.1089/15383440260682071
摘要
A new platform of hypothermic solutions, the HypoThermosol® (HTS) series, has been developed for the improved hypothermic storage of cells, tissues, and organs. Cells and tissues cold-stored in HTS-FRS demonstrate improved viability when returned to normothermic temperatures in comparison with the parent solution, HTS-BASE, or University of Wisconsin (UW) solution (UW-ViaSpan®). While our group and others have implicated apoptosis as a major player in cell death initiated by extended hypothermic storage, it has been unclear if the improved performance of HTS-FRS as a hypothermic storage solution is due to its ability to inhibit apoptosis. Data reported herein show that human renal cells hypothermically stored in renal cell culture medium, HTS-FRS, HTS-BASE, or UW solution demonstrated improved survival in HTS-FRS. Following 5 days of hypothermic preservation and 1 day of recovery at 37°C, cells preserved in HTS-FRS exhibit 75% metabolic activity, whereas cells stored in HTS-BASE, UW, or culture media demonstrate 32%, 17%, and 6% recovery, respectively. In addition, cells stored in HTS-BASE supplemented with caspase inhibitor exhibit increased cell numbers in comparison to cells stored in HTS-BASE (72% vs. 30% after 7 days of cold storage and 2 days of recovery). Experiments with annexin and propidium iodide as well as assessment of caspase activities suggest that the improved performance of HTS-FRS as a preservation solution may be due to its ability to inhibit both apoptosis and necrosis.
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