拉沙病毒
病毒学
病毒
拉沙热
灯1
生物
受体
外域
细胞内
病毒包膜
病毒进入
向性
细胞生物学
内体
病毒复制
遗传学
作者
Lucas T. Jae,Matthijs Raaben,Andrew S. Herbert,Ana I. Kuehne,Ariel S. Wirchnianski,Timothy K. Soh,Sarah H. Stubbs,Hans Janssen,Markus Damme,Paul Säftig,Sean P. J. Whelan,John M. Dye,Thijn R. Brummelkamp
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2014-06-27
卷期号:344 (6191): 1506-1510
被引量:250
标识
DOI:10.1126/science.1252480
摘要
Lassa virus spreads from a rodent to humans and can lead to lethal hemorrhagic fever. Despite its broad tropism, chicken cells were reported 30 years ago to resist infection. We found that Lassa virus readily engaged its cell-surface receptor α-dystroglycan in avian cells, but virus entry in susceptible species involved a pH-dependent switch to an intracellular receptor, the lysosome-resident protein LAMP1. Iterative haploid screens revealed that the sialyltransferase ST3GAL4 was required for the interaction of the virus glycoprotein with LAMP1. A single glycosylated residue in LAMP1, present in susceptible species but absent in birds, was essential for interaction with the Lassa virus envelope protein and subsequent infection. The resistance of Lamp1-deficient mice to Lassa virus highlights the relevance of this receptor switch in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI