G2-M DNA损伤检查点
支票1
蚜菌灵
细胞生物学
细胞周期蛋白依赖激酶1
细胞周期蛋白依赖激酶
有丝分裂
细胞周期检查点
细胞周期
DNA复制
DNA损伤
生物
癌症研究
DNA
遗传学
细胞
作者
Ning Xu,Silvana Libertini,Yaou Zhang,David A. Gillespie
标识
DOI:10.1016/j.bbrc.2011.08.119
摘要
It has been reported previously that both Cdk1 and Cdk2 phosphorylate Chk1 in a cell-cycle dependent manner. Cdk-mediated phosphorylation is required for efficient activation of Chk1 and checkpoint proficiency in response to DNA damage. Here, we demonstrate that Cdk-mediated phosphorylation is also required for replication stress induced Chk1 activation and S/M checkpoint proficiency. Re-introduction of Chk1 mutant (S286A/S301A) into Chk1 deficient cells is capable of restraining mitosis in cells with completely unreplicated DNA, but the mitotic delay at later stage of the cell cycle is largely impaired. The mutation strongly attenuates aphidicolin induced Chk1 activation without altering the S-phase dependent Chk1 activation. These data indicate that Cdk-mediated phosphorytion is required for efficient Chk1 activation and multiple checkpoint proficiency.
科研通智能强力驱动
Strongly Powered by AbleSci AI