清脆的
生物
基因组
病毒学
病毒
基因组编辑
遗传学
回文
计算生物学
基因
作者
Nicholas J. Baltes,Aaron W. Hummel,Eva Konečná,Radim Čegan,Aaron N. Bruns,David M. Bisaro,Daniel F. Voytas
出处
期刊:Nature plants
[Springer Nature]
日期:2015-09-28
卷期号:1 (10)
被引量:339
标识
DOI:10.1038/nplants.2015.145
摘要
To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.
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