Nutrients removal from low C/N actual municipal wastewater by partial nitritation/anammox (PN/A) coupling with a step-feed anaerobic-anoxic-oxic (A/A/O) system

In this study, a novel combined strategy was successfully established by partial nitritation/anammox (PN/A) within a step-feed A/A/O process integrated with fixed-biocarriers to treat municipal sewage for 200 days. The excellent nutrients removal performance of this system compared with national level of discharging standard were achieved: low total inorganic nitrogen (7.1 mg/L) and phosphorus (0.3 mg/L) in the effluent with the influent (51.1 and 4.2 mg/L) at C/N ratios of 3.4 ± 0.5, mainly attributed to the stable PN (oxic zone) and subsequently anammox effect (anoxic zone). Nitrogen mass balance indicated that anammox contribution in anoxic zones to nitrogen loss could be up to 42% at stable phase. Therefore, aeration and carbon cost could be greatly reduced under low DO, low C/N and aerobic hydraulic retention time (HRT) of 7.4 h condition. The low DO and anammox bacteria retention in anoxic chambers promoted the washout of NOB and combination of anammox and partial nitritation process. During long-term operation, the activity of AOB effectively maintained while that of NOB drastically reduced to 0.1 mg N / g MLSS / h resulting in high and stable nitrite accumulation ratios (about 90%). The achievement of partial nitritation was mainly due to low DO (0.4–0.5 mg/L) and effective retention of anammox bacteria even with a low temperature (14.5 °C). Notedly, anammox activity gradually increased both on the biocarriers and in the flocs while a higher anammox abundance was observed on the biocarriers (2.48%) than that in suspend flocs (0.03%). As above, this study indicated that the novel combined strategies could be applicable to mainstream anammox, and a pilot-scale reactor will be established to verify and promote the industrial application of mainstream anammox. • The PN/A achieved with NAR of 90% and anammox contributed 42% N-loss in mainstream. • The TN and P in influent and effluent were 51.1, 4.2 and 7.1, 0.3 mg/L at C/N of 3.4. • NOB was repressed with activity decreased to non-active at abundance of 0.11%. • Anammox bacteria maintained high abundance on carriers and flocs at 2.48% and 0.03%.