猪流行性腹泻病毒
菲咯烷
病毒学
生物
维罗细胞
病毒
热休克蛋白70
病毒复制
寄主(生物学)
微生物学
热休克蛋白
基因
生物化学
遗传学
作者
Duo‐Zhi Chen,Shi-Rui Fan,Bi-juan Yang,Huochun Yao,Yi‐Ting Wang,Jie‐Yun Cai,Chenxu Jing,Zihao Pan,Miao Luo,Yan-Qiu Yuze,Guangjin Liu,Xiao‐Jiang Hao
标识
DOI:10.1021/acs.jnatprod.0c01252
摘要
Porcine epidemic diarrhea virus (PEDV) has become increasingly problematic around the world, not only for its hazards to livestock but also due to the possibility that it is a zoonotic disease. Although vaccine therapy has made some progress toward PEDV control, additional effective therapeutic strategies against PEDV are needed, such as the development of chemotherapeutic agents. The aim of this work was to identify novel anti-PEDV agents by designing and synthesizing a series of phenanthridine derivatives. Among them, three compounds (compounds 1, 2, and 4) were identified as potent anti-PEDV agents exhibiting suppression of host cell heat shock cognate 70 (Hsc70) expression. Mechanism studies revealed that host Hsc70 is involved in the replication of PEDV, and its expression can be suppressed by destabilization of the mRNA, resulting in inhibition of PEDV replication. Activity against PEDV in vivo in PEDV-infected piglets suggested that phenanthridine derivatives are the first host-acting potential anti-PEDV agents.
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