Isolation and Identification of Yue A Strain of Pseudorabies Virus

An isolate suspected to be pseudorabies virus was recovered from dead piglets on a pig farm in Panyu Guangdong. It was characterized by its physicochemical properties, virus neutralization test, animal inoculation, electron microscopy, PCR amplification and in vitro infectivity test. The isolate was found to be sensitive both to chloroform and ether, could be neutralized by the standard pseudorabies Fujian A strain virus antibodies, rabbits inoculated with the isolate developed localized itch and then paralysis before death, electron microscope revealed irregularly round virus particles with envelope and peplomers, PCR amplification of the virus genome yielded a fragment which co migrated with similarly amplified fragment of the Fujian A standard PRV. When titrated in Marc 145 cell culture, the virus titre attained 10 7.5 TCID 50 /mL. The isolate was identified as a pseudorabies virus on the strength of the above findings.