检出限
适体
再现性
荧光
自来水
量子点
纳米颗粒
化学
选择性
色谱法
纳米技术
线性范围
材料科学
环境科学
分子生物学
生物
生物化学
环境工程
物理
量子力学
催化作用
作者
Qingyi Wei,Peiyao Zhang,Hongbin Pu,Da‐Wen Sun
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-03-01
卷期号:373: 131591-131591
被引量:33
标识
DOI:10.1016/j.foodchem.2021.131591
摘要
Trace amounts of 17β-estradiol (E2) in food and the environment poses a threat to human health, which has created the demand for sensitive analytical methods to detect E2. In this study, a novel fluorescent aptasensor was developed for sensitive detection of E2 based on double-chain hybridization between carbon quantum dots-labelled with E2 aptamer (CQDs-aptamer) and Fe3O4 nanoparticles modified by complementary DNA (Fe3O4-cDNA). Under the optimal conditions, the aptasensor displayed a good linear range of 10-11-10-6 M for E2 with the coefficient of determination (R2) of 0.996, and a low detection limit of 3.48 × 10-12 M was obtained. Besides, the aptasensor showed high selectivity and good reproducibility for E2 detection, which was successfully applied to the sensitive detection of E2 in milk as compared with tap water and lake water with satisfactory recoveries from 85.21% to 114.80%, suggesting the great significance of this aptasensor for detecting food contaminants in the food industry.
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