Helicobacter pylori is one of the most common bacterial pathogens in humans. The infection with these bacteria is now recognized as a worldwide problem. It causes chronic gastritis, peptic ulcer disease, and lymphoproliferative disorders and is a major risk factor for gastric cancer. The aim of this research was to investigate the gastric disorders from dogs with spontaneous Helicobacter pylori infection by necropsy and histopathological exams, also identification of the Helicobacter pylori bacteria by immunohistochemistry method in tissues with lesions. At necropsy and histopatological exams we found more specific lesions of the Helicobacter species infection, respectively Helicobacter pylori infection in dogs. The most common types of specific gastritis were chronic follicular gastritis, fibrous gastritis and ulcerative gastritis. The lesions was main present in pyloric and fundic areas. At two cases was not observed lesions. Detection of Helicobacter pylori bacteria has been accomplished by immunohistochemistry method using policlonal antibody