转录因子
基因亚型
生物
抄写(语言学)
基因
响应元素
分子生物学
发起人
基因表达
拟南芥
反作用
遗传学
细胞生物学
突变体
语言学
哲学
作者
Hyeong Cheol Park,Man Lyang Kim,Yun Hwan Kang,Joo Mi Jeon,Jae Hyuk Yoo,Min Chul Kim,Chan Young Park,Jae Cheol Jeong,Byeong Cheol Moon,Ju Huck Lee,Hae Won Yoon,Sungho Lee,Woo Sik Chung,Chae Oh Lim,Sang Yeol Lee,Jong Chan Hong,Moo Je Cho
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2004-08-01
卷期号:135 (4): 2150-2161
被引量:341
标识
DOI:10.1104/pp.104.041442
摘要
Abstract The Ca2+-binding protein calmodulin mediates cellular Ca2+ signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter sequence of the gene. Deletion analysis of the promoter revealed that a 130-bp region located between nucleotide positions −858 and −728 is required for the stressors to induce expression of SCaM-4. A hexameric DNA sequence within this region, GAAAAA (GT-1 cis-element), was identified as a core cis-acting element for the induction of the SCaM-4 gene. The GT-1 cis-element interacts with an Arabidopsis GT-1-like transcription factor, AtGT-3b, in vitro and in a yeast selection system. Transcription of AtGT-3b is also rapidly induced within 30 min after pathogen and NaCl treatment. These results suggest that an interaction between a GT-1 cis-element and a GT-1-like transcription factor plays a role in pathogen- and salt-induced SCaM-4 gene expression in both soybean and Arabidopsis.
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