数字聚合酶链反应
邻近连接试验
信使核糖核酸
寡核苷酸
化学
分子生物学
细胞
细胞生物学
出处
期刊:Methods in molecular biology
日期:2021-11-12
卷期号:: 157-169
标识
DOI:10.1007/978-1-0716-1771-7_11
摘要
Digital proximity ligation assay (PLA) detects single protein molecules with a pair of oligonucleotide-conjugated antibodies and digital PCR (dPCR) readout, which allows absolute quantitation of proteins in single cells with high sensitivity. The pipeline also allows simultaneous measurement of protein and mRNA from the same single cell. The sensitivity of the assay has been further improved with implementation of the assay on a microfluidic system, which enables quantitation of rare protein species, with expression level as low as ~3000 protein molecules per cell.
科研通智能强力驱动
Strongly Powered by AbleSci AI