环介导等温扩增
疟疾
恶性疟原虫
孔雀绿
生物
间日疟原虫
酚红
疟疾疟原虫
疟原虫(生命周期)
血凝蛋白
色谱法
病毒学
寄生虫寄主
生物化学
化学
免疫学
DNA
计算机科学
有机化学
吸附
遗传学
万维网
作者
Lee Phone Youth Zen,Meng-Chuan Lai,Syaza Izzati binti Rozlan,Mohd. Kamaruddin Abd. Hamid,Jenarun Jelip,Rose Nani Mudin,Yee Ling Lau
标识
DOI:10.1016/j.exppara.2022.108310
摘要
In order to ascertain the results of the LAMP technique, different end-point detection methods can be employed. However, these methods require sophisticated equipment. To simplify current end-point detection methods for the diagnosis of malaria, we propose the incorporation of colorimetric dyes: malachite green (MG), phenol red (PR), and xylenol orange (XO) in the LAMP assay. To evaluate the optimum concentration of dyes, 5 different concentrations (50 μM, 75 μM, 100 μM, 125 μM, and 150 μM) were used with buffer pH 8.5 and pH 8.8, respectively. The results showed that 125 μM of MG at pH 8.8 produced the most obvious colour change. A total of 71 clinical blood samples of Plasmodium knowlesi, Plasmodium malariae, Plasmodium vivax, Plasmodium falciparum, and healthy donors were tested using MG-LAMP. It showed 100% sensitivity and specificity. The simplicity and affordability of this method make it ideal to be used as an end-point detection method for malaria diagnosis in resource limited settings.
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