多糖
免疫系统
抗氧化剂
甘露糖
乙醇
化学
乙醇沉淀
石斛
萃取(化学)
甘露聚糖
生物化学
谷胱甘肽
食品科学
生物
酶
免疫学
植物
色谱法
作者
Sujun Sun,Peng Deng,Chune Peng,Haiyu Ji,Longfei Mao,Lizeng Peng
出处
期刊:Polymers
[MDPI AG]
日期:2022-07-16
卷期号:14 (14): 2899-2899
被引量:69
标识
DOI:10.3390/polym14142899
摘要
The ethanol precipitation method has been widely-used for Dendrobium officinale polysaccharides preparation. However, the alcohol-soluble fractions have always been ignored, which causes significant wastes of resources and energies. In this study, the extraction, physicochemical properties, and immune regulation activity of an edible D. officinale polysaccharide (DOPs) isolated from the supernatant after 75% ethanol precipitation were systematically investigated. The structural characteristics determination results showed that DOPs was mainly composed of glucose and mannose at a molar ratio of 1.00:5.78 with an average molecular weight of 4.56 × 103 Da, which was made up of α-(1,3)-Glcp as the main skeleton, and the α-(1,4)-Glcp and β-(1,4)-Manp as the branches. Subsequently, the cyclophosphamide (CTX)-induced immunosuppressive mice model was established, and the results demonstrated that DOPs could dose-dependently protect the immune organs against CTX damage, improve the immune cells activities, and promote the immune-related cytokines (IL-2, IFN-γ and TNF-α) secretions. Furthermore, DOPs treatment also effectively enhanced the antioxidant enzymes levels (SOD, GSH-Px) in sera and livers, therefore weakening the oxidative damage of CTX-treated mice. Considering these above data, DOPs presented great potential to be explored as a natural antioxidant and supplement for functional foods.
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