自噬
姜黄素
糖尿病性心肌病
安普克
细胞凋亡
体内
糖尿病
药理学
体外
链脲佐菌素
医学
化学
细胞生物学
心肌病
内科学
内分泌学
心力衰竭
生物
磷酸化
蛋白激酶A
生物化学
生物技术
作者
Qing Yao,Zhiqiang Ke,Shuang Guo,Xiaosong Yang,Fei-xue Zhang,Xiufen Liu,Chen Xiao,Hongguang Chen,Huan-ya Ke,Chao Liu
标识
DOI:10.1016/j.yjmcc.2018.10.004
摘要
The effects of curcumin on regulating cardiac apoptosis and autophagy were analyzed in diabetic models both in vivo and in vitro. In vivo, experimental diabetes was induced in mice by low-dose STZ injection combined with a high-fat diet. In vitro, cultured H9c2 cardiomyoblasts were exposed to high d-glucose concentrations combined with palmitate. Our results showed that apoptosis was increased and autophagy was suppressed in the hearts of diabetic mice, which was ameliorated by curcumin treatment, ultimately improving cardiac function. Moreover, the inhibition of autophagy exacerbated apoptotic death in cardiac cells under diabetic condition. Curcumin activated AMPK and JNK1, which phosphorylated Bcl-2 and Bim and subsequently disrupted their interactions with Beclin1, thereby promoting autophagy and alleviating apoptosis respectively. In addition, AMPK-mediated inhibition of mTORC1 pathway likely played a role in regulating autophagy by curcumin under diabetic condition. Our study suggests that curcumin protects against diabetic cardiomyopathy by modulating the crosstalk between autophagic and apoptotic machinery. Modulation of autophagy may be an effective strategy for the treatment of cardiovascular diseases associated with diabetes.
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