OBJECTIVE: To explore the effect of comprehensive intervention on the diabetes-relevant behaviors in aging patients with prediabetes in rural areas of Yiyang. Methods: The aging patients with prediabetes in rural areas of Yiyang were chosen and asked for the questionnaire. The patients were randomly divided into two groups: the control group and the intervention group. The control group received the routine health education, while the intervention group underwent comprehensive intervention for one year. 6 months and 12 months before the intervention or after the intervention, the behavior characteristics of diabetes, levels of related physiological and biochemical indexes were evaluated. Results: The relative behavior characteristics of diabetes, levels of related physiological and biochemical indexes between the two groups were comparable before the intervention (P>0.05). 6 months or 12 months after the intervention, it showed improvements in diabetes-related behaviors (P<0.05) and the scores of diabetes-related behaviors compared with the control group (P<0.001). Based on the related physiological and biochemical indexes, the intervention group showed significant reductions in the levels of waist-to-hip ratio, BMI, fasting plasma glucose and 2 h postprandial plasma glucose (P<0.01). Conclusion: The comprehensive intervention can improve the diabetes-relevant behaviors, physiological and biochemical indexes in the older patients with pre-diabetes.