CHARACTERIZATION AND PURIFICATION OF A GLUCOSE-6-PHOSPHATE DEHYDROGENASE VARIANT Gd (-) ZHANJIANG IN HUMAN ERYTHROCYTES

A human glucose-6-phosphate dehydrogenase variant was characterized and purified. The result showed that this was a new variast which was named as Gd (-) Zhanjiang. Compared with the specific activity (163IU/mg) of the purified normal glucose-6-phosphate dehydrogenase, the specif ic activity (28IU/mg) of the purified Gd (-) Zhanjiang was lower than that of normal one and indicated that decrease in enzyme activity was the major defect of this new glucose-6-phosphate dehydrogenase variant. Key words: Glucose-6-phosphate dehydrogenase; purification; structure and function of enzyme; glucose-6-phosphate dehydrogenase variant