材料科学
荧光寿命成像显微镜
光学
断层摄影术
荧光
物理
作者
Veronica C. Torres,Chengyue Li,Wei Zhou,Jovan G. Brankov,Kenneth M. Tichauer
出处
期刊:Applied Optics
[The Optical Society]
日期:2020-12-22
卷期号:60 (1): 135-135
被引量:10
摘要
Transmittance and fluorescence optical projection tomography can offer high-resolution and high-contrast visualization of whole biological specimens; however, applications are limited to samples exhibiting minimal light scattering. Our previous work demonstrated that angular-domain techniques permitted imaging of ∼ 1 c m diameter noncleared lymph nodes because of their low scattering nature. Here, an angle-restricted transmittance/fluorescence system is presented and characterized in terms of geometric and fluorescence concentration reconstruction accuracy as well as spatial resolution, depth of focus, and fluorescence limits of detection. Using lymph node mimicking phantoms, results demonstrated promising detection and localization capabilities relevant for clinical lymph node applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI