化学
色谱法
甘草
四极飞行时间
质谱法
高效液相色谱法
根茎
串联质谱法
传统医学
医学
病理
替代医学
作者
Tongwen Xu,Min Yang,Yifeng Li,Xin Chen,Qiurong Wang,Wangping Deng,Xiayan Pang,Kate Yu,Baohong Jiang,Shu‐Hong Guan,Dan Guo
摘要
RATIONALE Licorice (Gancao) is derived from the dried roots and rhizomes of Glycyrrhiza species (Leguminosae) and appears as a component herb in about 60% of traditional Chinese medicine (TCM) prescriptions. Modern pharmacological studies have shown that flavonoids are one class of the major components responsible for the bioactivities of licorice. Ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UPLC/QTOF MS) has proven to be a powerful tool for rapid profiling and identification of natural products in complex herbal medicines. METHODS A UPLC/QTOF MS method was established for the first time for profiling and structural characterization of the phenolic compounds (most of them flavonoids) in licorice. The combined use of data‐independent acquisition (MS E ) and data‐dependent acquisition (DDA) was illustrated. RESULTS Fifteen flavonoid reference compounds were used to explore the fragmentation pathways. Compound identification was based upon the exact mass, general fragmentation behaviors, retention times, UV absorption, and the related botanical biogenesis. As a result, a total of 51 compounds were characterized, three of which were reported for the first time. CONCLUSIONS The LC/MS analysis for each injection took less than 9 min. The developed method is fast, accurate and reliable due to its high resolution and high efficiency characteristics as a result of combining both UPLC separation and QTOF exact mass measurement. Copyright © 2013 John Wiley & Sons, Ltd.
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