纳米纤维
基质凝胶
聚乙烯醇
诱导多能干细胞
化学
细胞培养
干细胞
细胞生物学
细胞
纳米技术
材料科学
生物化学
生物
胚胎干细胞
有机化学
基因
遗传学
作者
Yi Deng,Xiaohong Zhang,Yinghui Zhao,Shangshang Liang,Anxiu Xu,Xiang Gao,Feng Deng,Jing Fang,Shicheng Wei
标识
DOI:10.1016/j.carbpol.2013.09.030
摘要
Realization of the full potential of human induced pluripotent stem cells (hiPSCs) in clinical applications requires development of well-defined conditions for their growth and differentiation. A novel fully defined polyvinyl alcohol/hyaluronan (PVA/HA) polysaccharide nanofiber was developed for hiPSCs culture in commercially available xeno-free, chemically defined medium. Vitronectin peptide (VP) was immobilized to PVA/HA nanofibers through NHS/EDC chemistry. The hiPSCs successfully grew and proliferated on the VP-decorated PVA/HA nanofibers, similar to those on Matrigel™. Such well-defined, xeno-free and safe nanofiber substrate that supports culture of hiPSCs will not only help to accelerate the translational perspectives of hiPSCs, but also provide a platform to investigate the cell–nanofiber interaction mechanisms that regulate stem cell proliferation and differentiation.
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