MyoD-cre transgenic mice: A model for conditional mutagenesis and lineage tracing of skeletal muscle

genesisVolume 41, Issue 3 p. 116-121 Technology Report MyoD-cre transgenic mice: A model for conditional mutagenesis and lineage tracing of skeletal muscle Jennifer C. J. Chen, Jennifer C. J. Chen Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutSearch for more papers by this authorJustin Mortimer, Justin Mortimer Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutSearch for more papers by this authorJason Marley, Jason Marley Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutSearch for more papers by this authorDavid J. Goldhamer, Corresponding Author David J. Goldhamer david.goldhamer@uconn.edu Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutCenter for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, 1392 Storrs Road, Unit 4243, Storrs, CT 06269-4243Search for more papers by this author Jennifer C. J. Chen, Jennifer C. J. Chen Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutSearch for more papers by this authorJustin Mortimer, Justin Mortimer Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutSearch for more papers by this authorJason Marley, Jason Marley Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutSearch for more papers by this authorDavid J. Goldhamer, Corresponding Author David J. Goldhamer david.goldhamer@uconn.edu Center for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, Storrs, ConnecticutCenter for Regenerative Biology, Department of Molecular and Cell Biology, University of Connecticut, 1392 Storrs Road, Unit 4243, Storrs, CT 06269-4243Search for more papers by this author First published: 07 March 2005 https://doi.org/10.1002/gene.20104Citations: 44AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The Cre-loxP recombination system has been used to great advantage in vivo for conditional gene targeting, lineage tracing, and other applications. To express cre in skeletal myoblasts and muscle fibers, we utilized the well-characterized transcriptional regulatory regions of the muscle determination gene, MyoD. Transgenic mouse lines were produced (F3/-2.5cre) in which the cre gene is driven by the MyoD promoter and core enhancer, which directs the early activation of MyoD. Specificity of cre expression and efficiency of recombination was determined by monitoring reporter gene expression after crossing to the Cre-dependent reporter lines, R26R and Z/AP. Efficient labeling of embryonic and fetal myoblasts and muscle fibers was observed, with timing that was similar (branchial arches and limb buds) or slightly delayed (myotomes) relative to the endogenous MyoD gene. In satellite cell cultures, a strict concordance between MyoD protein and reporter gene expression was observed, demonstrating the muscle specificity and efficiency of Cre-mediated recombination. Nascent muscle fibers were labeled following injury of adult muscle, indicating recombination in satellite cells or their daughters in vivo. genesis 41:116–121, 2005. © 2005 Wiley-Liss, Inc. Citing Literature Volume41, Issue3March 2005Pages 116-121 RelatedInformation