Advances in Fluorescence-based Probes for Abiotic Stress Detection in Plants

Abiotic stress poses significant challenges to the ecological environment and global food security. Early and accurate diagnosis of abiotic stress is essential for modern agriculture. Recently, fluorescence sensing technology has emerged as a valuable tool for monitoring abiotic stress due to its ease of use and capability for spatiotemporal visualization. These probes specifically bind to abiotic stress biomarkers, facilitating the detection of stress responses and advancing related biological research. However, there is a lack of comprehensive reviews on fluorescence probe for abiotic stress, which limits progress in this area. This review outlines the biological markers of abiotic stress, discusses the types and design principles of fluorescence probe, and reviews research on detecting plant responses to such stress. Its goal is to inspire the rational design of fluorescence probe for plant bioimaging, promote early diagnosis of abiotic stress, and enhance the understanding of plant defense mechanisms at the molecular level, ultimately providing a scientific basis for stress management in agriculture.