检出限
石墨烯
线性范围
胶体金
二硫化钨
适体
材料科学
纳米技术
电化学
免疫分析
组合化学
甲状腺癌
化学
纳米颗粒
色谱法
抗体
甲状腺
分子生物学
电极
物理化学
冶金
医学
内科学
免疫学
生物
作者
Wenjing Wang,Huabiao Tang,Lili Zhou,Zhaohui Li
出处
期刊:Molecules
[MDPI AG]
日期:2024-01-22
卷期号:29 (2): 552-552
标识
DOI:10.3390/molecules29020552
摘要
Thyroid transcription factor 1 (TTF1) is an important cancer-related biomarker for clinical diagnosis, especially for carcinomas of lung and thyroid origin. Herein, a novel label-free electrochemical immunosensor was prepared for TTF1 detection based on nanohybrids of ribbon-like tungsten disulfide-reduced graphene oxide (WS2-rGO) and gold nanoparticles (AuNPs). The proposed immunosensor employed H2O2 as the electrochemical probe because of the excellent peroxidase-like activity of ribbon-like WS2-rGO. The introduction of AuNPs not only enhanced the electrocatalytic activity of the immunosensor, but also provided immobilization sites for binding TTF1 antibodies. The electrochemical signals can be greatly amplified due to their excellent electrochemical performance, which realized the sensitive determination of TTF1 with a wide linear range of 0.025–50 ng mL−1 and a lower detection limit of 0.016 ng mL−1 (S/N = 3). Moreover, the immunosensor exhibited high selectivity, good reproducibility, and robust stability, as well as the ability to detect TTF1 in human serum with satisfactory results. These observed properties of the immunosensor enhance its potential practicability in clinical applications. This method can also be used for the detection of other tumor biomarkers by using the corresponding antigen–antibody complex.
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