乳状液
佐剂
壳聚糖
粒径
免疫系统
粒子(生态学)
材料科学
化学
乙醇酸
化学工程
色谱法
乳酸
免疫学
医学
生物
生物化学
生态学
遗传学
物理化学
细菌
工程类
作者
Lanxin Li,Yanan Li,Yanan Peng,Guanghui Ma,Jie Wu
标识
DOI:10.1016/j.intimp.2023.111468
摘要
Particle-in-oil-in-water (P/O/W) multiple emulsion adjuvants introduce particles into the internal water phase of a water-in-oil-in-water emulsion, combining the advantages of both particle and emulsion adjuvants to enhance humoral and cellular immune responses. In this study, we optimized P/O/W multiple emulsion adjuvants. Chitosan, poly (lactic-co-glycolic acid), and aluminum gel were used to prepare the particles, which were introduced into a water-in-oil-in-water emulsion to obtain three P/O/W multiple emulsion adjuvants. The immune enhancement effects and safety of the three adjuvants were compared, and it was proven that the adjuvant with chitosan nanoparticles in the internal water phase had good cellular and humoral immune effects. Simultaneously, the proportion of the internal water phase increased from 13% to 20%, reducing the antigen concentration required for embedding to one-third of the original concentration and expanding the application range of the composite adjuvant.
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