轨道轨道
质谱法
定量蛋白质组学
频谱分析仪
化学
分光计
蛋白质组学
计算机科学
色谱法
物理
光学
电信
生物化学
基因
作者
Lilian R. Heil,Nicolaie Eugen Damoc,Tabiwang N. Arrey,Anna Pashkova,Eduard Denisov,J. Petzoldt,Amelia C. Peterson,Chris Hsu,Brian C. Searle,Nicholas Shulman,Michael Riffle,B. Connolly,Brendan MacLean,Philip M. Remes,Michael W. Senko,H. W. Stewart,Christian Hock,Alexander Makarov,Daniel J. Hermanson,Vlad Zabrouskov,Christine C. Wu,Michael J. MacCoss
标识
DOI:10.1101/2023.06.03.543570
摘要
We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data independent acquisition, the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Thermo Scientific™ Orbitrap™ mass spectrometers, which have long been the gold standard for high resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer can produce high quality quantitative measurements across a wide dynamic range. We also use a newly developed extra-cellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5,000 plasma proteins in a 60-minute gradient with the Orbitrap Astral mass spectrometer.
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