This study delves into the transformative impact of diverse nutrient management practices on the yield, quality, soil health and carbon sequestration potential of multi-cut fodder sorghum. Employing a randomized block design with three replications across 11 treatments, the research evaluated the effects of organic, inorganic and biofertilizer-based nutrient applications. The findings were striking: a combination of poultry manure at 75 % nitrogen equivalent with biofertilizers (Arbuscular mycorrhiza and Azophos) led to a remarkable 31.2 % boost in fodder yield and a 36.4 % increase in dry matter production compared to the control. Soil analysis revealed an 8.22 % decrease in pH, alongside substantial enhancements in key nutrients, with available nitrogen, phosphorus and potassium levels soaring by 28.40 %, 44.19 % and 9.43 % respectively, under the same treatment. These practices also supercharged soil enzyme activities, with notable increases in amylase (1.21 mg reducing sugars g-1 soil 24 h-1), invertase (1.45 mg reducing sugars g-1 soil 24 h-1), cellulase (34.8 µg reducing sugars g-1 soil 24 h-1), phosphatase (19.73 µg phenols g-1 soil 24 h-1), dehydrogenase (11.56 µg triphenyl formazan g-1 soil 24 h-1) and urease (17.92 mg NH4 - N g-1 soil 24 h-1), signaling a vibrant uptick in microbial activity and overall soil health. Moreover, this nutrient strategy delivered a 39.22 % rise in soil organic carbon (SOC) and a 38.74 % boost in soil carbon stock, highlighting its powerful potential for long-term carbon sequestration. This study offers critical insights for crafting sustainable agricultural practices that not only maximize crop production but also enhance soil fertility and contribute meaningfully to environmental conservation.