色谱法
肉桂酸
化学
磷酸
药代动力学
分析物
生物利用度
高效液相色谱法
检出限
萃取(化学)
药理学
生物化学
医学
有机化学
抗氧化剂
作者
Burhan Ceylan,Gizem Tırıs,Şerife Evrim Kepekçi Tekkeli
出处
期刊:Journal of Chromatographic Science
[Oxford University Press]
日期:2023-02-24
卷期号:62 (1): 21-26
标识
DOI:10.1093/chromsci/bmad005
摘要
An HPLC method with UV detection was developed for the determination of carnosic acid in human plasma and applied to a pharmacokinetic study after oral administration of Rosemary extract to a healthy volunteer. Sample preparation depends on liquid-liquid extraction with hexane. Chromatographic separation was achieved with C18 column (150 mm × 4.6 mm × 5 μm), at 25°C with isocratic elution, mobile phase composed of solution A (methanol), and solution B (2% o-phosphoric acid in water) (90:10, v/v) at flow rate of 1.0 mL/min. The analyte was detected at 230 nm. The retention time is 4.20 ± 0.03 min. The method was validated in terms of accuracy, precision, specificity, robustness and detection and quantification limits, in accordance with European Medicines Agency guidelines. LOD and LOQ were found to be 0.075 and 0.25 ng/mL, respectively. The method was applied to the analysis of carnosic acid in human plasma with good recovery as 91.7%. The plasma concentration-time profile and pharmacokinetic parameters: AUC0-t, AUC0-∞, Cmax, tmax, t1/2 were calculated according to the assays. The method can certainly be used for routine analysis of carnosic acid in human plasma after oral administration of Rosemary extract, and for phase I clinical studies and bioavailability-bioequivalance studies as well.
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