[Objective] To approach the effective standardized management for cleaning and disinfection of endoscope, in other to prevent medical infection by using endoscope examination, enhance the medical care and assure the medical safety. [Methods] The results of random check up the cleaned and disinfected endoscopes and their accessories were reviewed that be ready by using NCX12-III automatic ultrasonic nebulization endoscopecleaning and disinfecting machine(2% glutaric dialdehyde) to treat-artificial cleaning endoscope during Jan. 2005 and Dec. 2006. According to the check up methods, the same investingated samples were divided into group A (endoscope inner sampling) and group B (endoscope surface sampling). [Results] 47 pieces of endoscope were investigated and samples 94 pieces were picked, each group 47 pieces. All endoscopes in group A won 100% qualification rate, but there were two samples of group B in excess of clony numbers, the sum qualification rate was 95.74%, with the gastroscope 94.74%, enteroscope 92.31%, and bronchofibroscope 100%. [Conclusion] The standardized management for cleaning and disinfection of endoscope is the most important key to assure disinfection quality of endoscopes. Both endoscope inner sampling and surface sampling methods are suggested on detection and check up the effect of disinfection of endoscope, to correctly valuate the cleaned and disinfected quality of endoscope.