The Significance of Expression Angs/Tie-2 System in Myeloid Leukemia

Objective:To investigate the expression level of Angs/Tie-2 and VEGF mRNA and their significance in myeloid leukemia.Methods:The expression of Ang-1,Ang-2,Tie-2 and VEGF mRNA in 80 AML patients,10 CML patients and 10 healthy controls were detected by RT-PCR.The Proliferation Index(PI) of leukemia cells was assayed by flow cytometry(FCM).Results: The expression levels of Ang-2,Tie-2 and VEGF mRNA,except Ang-1 mRNA,were statistically significant higher in untreated myeloid leukemia patients than those in complete remission(CR) patients.The percentage of extra-medullary infiltrations were significant higher in both Ang-2 and VEGF positive group(P0.05).Patients with both Ang-2 and Tie-2 positive had a lower CR rate(P0.05) but a higher PI(P0.05).The expression levels of Ang-2 and Tie-2 increased significantly with the progressing of CML.Conclusion:Angs/Tie-2 mRNA are over-expressed in myeloid leukemia.The over-expressed Angs/Tie-2 may have synergistic effect with VEGF in the pathogenesis of leukemia.