透明质酸
间充质干细胞
自愈水凝胶
骨形态发生蛋白2
生物医学工程
材料科学
组织工程
骨钙素
细胞生物学
再生(生物学)
化学
解剖
体外
生物化学
碱性磷酸酶
生物
高分子化学
医学
酶
作者
Jung‐Ju Kim,In Sook Kim,Tae Hyung Cho,Kyu Back Lee,Soon Jung Hwang,Giyoong Tae,Insup Noh,Sang Hoon Lee,Yongdoo Park,Kyung Sun
出处
期刊:Biomaterials
[Elsevier]
日期:2007-04-01
卷期号:28 (10): 1830-1837
被引量:465
标识
DOI:10.1016/j.biomaterials.2006.11.050
摘要
Acrylated hyaluronic acid (HA) was used as a scaffold for bone morphogenic protein-2 (BMP-2) and human mesenchymal stem cells (hMSCs) for rat calvarial defect regeneration. HA was acrylated by two-step reactions: (1) introduction of an amine group using adipic acid dihydrazide (ADH); (2) acrylation by N-acryloxysuccinimide. Tetrathiolated poly(ethylene) glycol (PEG-SH(4)) was used as a cross-linker by a Michael-type addition reaction and the hydrogel was formed within 10min under physiological conditions. This hydrogel is degraded completely by 100U/ml hyaluronidase in vitro. hMSCs and/or BMP-2 was added during gelation. Cellular viability in vitro was increased up to 55% in the hydrogels with BMP-2 compared with the control. For in vivo calvarial defect regeneration, five different samples (i.e., control, hydrogel, hydrogel with BMP-2, hydrogel with MSCs, and hydrogel with BMP-2 and MSCs) were implanted for 4 weeks. The histological results demonstrated that the hydrogels with BMP-2 and MSCs had the highest expression of osteocalcin and mature bone formation with vascular markers, such as CD31 and vascular endothelial growth factors, compared with the other samples. This study demonstrated that HA base hydrogel can be used for cell and growth factor carriers for tissue regeneration.
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