Gold and platinum bimetallic nanoparticles with double enzyme-like properties for the colorimetric detection of glutathione

• Capreomycin was successfully used to prepare Cap@AuPt NPs with a particle size of 110 nm by biomineralization . • Cap@AuPt NPs possessed two types of enzyme-like activities: peroxidase-like activity and oxidase-like activity • The glutathione detection methods established by the two enzyme-like activities of Cap@AuPt NPs had good sensitivity. • The detection range and limit of detection of Cap@AuPt NPs for glutathione detection were 10-250 μM and 0.58 μM, respectively. Noble metal nanomaterials based nanozymes have been drawing great interest due to their wide application in the catalytic detection and nanomedicine. Herein, 110 nm capreomycin stabilized gold and platinum bimetallic nanoparticles (Cap@AuPt NPs) were prepared by biomineralization method with polypeptide capreomycin as a template, HAuCl 4 and K 2 PtCl 4 as precursors. The results showed that Cap@AuPt NPs had two kinds of enzyme activities: peroxide-like activity and oxidase-like activity. The catalytic kinetics of Cap@AuPt NPs was consistent with the Michaelis-Menten equation. A novel colorimetric assay for glutathione (GSH) was developed based on the outstanding catalytic activity of Cap@AuPt NPs with a detection range of 10–250 μM and a detection limit of 0.58 μM. Overall, these results demonstrated that Cap@AuPt NPs had peroxidase-like and oxidase-like ability and showed their good prospect in glutathione detection. 2 In this paper, capreomycin was used as a template, HAuCl 4 and K 2 PtCl 4 were used as precursors to prepare gold and platinum bimetallic nanoparticles (Cap@AuPt NPs). Compared with the catalytic activity of single metal nanoparticles, Cap@AuPt NPs had enhanced catalytic activity and improved accuracy and sensitivity of colorimetric determination of GSH.