Multimeric, multivalent fusion carrier proteins for site-selective glycoconjugate vaccines simultaneously targeting Staphylococcus aureus and Pseudomonas aeruginosa

A 3-in-1 glycoconjugate vaccine, incorporating multiple copies of three antigens from Staphylococcus aureus and Pseudomonas aeruginosa into one construct, was generated, leveraging a histidine-directed conjugation, and elicited a strong immune response against each antigen in mice.